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sv40 dna virus  (ATCC)


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    ATCC sv40 dna virus
    Sv40 Dna Virus, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 2806 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sv40+dna+atcc/TIBx%3B+Epithelial+liver%3B+Mouse/pmc10889654-59-12-18
    Average 97 stars, based on 2806 article reviews
    sv40 dna virus - by Bioz Stars, 2026-10
    97/100 stars

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    Transfection:

    Article Title: Enhanced Responses to Angiogenic Cues Underlie the Pathogenesis of Hereditary Hemorrhagic Telangiectasia 2
    Article Snippet: .. For immortalization when the cells reached 50% confluency, they were transfected with SV40 DNA: ATCC (VRMC-3), pUCSV40-B2E , , by using Lipofectamine (Invitrogen) according to the manufacturer’s protocol. ..

    Article Title: SMAD1 Deficiency in Either Endothelial or Smooth Muscle Cells Can Predispose Mice to Pulmonary Hypertension
    Article Snippet: .. When cells in the culture reached about 50% confluency post-isolation, they were transfected with 4.0 μg of SV40 DNA: ATCC (VRMC-3), pUCSV40-B2E3 using Lipofectamine (Invitrogen) following the manufacturer’s protocol. .. After a couple of passages, endothelial cells were sorted out by Fluorescence-Activated Cell Sorting (FACS) using Dio-Ac-LDL (Biomedical Technologies, Inc.) and lectin (Sigma-Aldrich Co.).



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    FIGURE 1 – Standard curves for quanti- tative real-time PCR (TaqMan) assays for <t>SV40</t> large T antigen, EBV-BKRF-1 (EBNA-1), human K-ras and t(14;18). Different linearized plasmid DNAs con- taining the cloned target DNA fragments or the complete SV40 viral genome were 2-fold serial diluted (105 copies to <1 copy) and 3 replicates were tested. Mean cycle threshold values (CT) of 3 replicate tests were plotted against the log copy number of each gene or the cloned viral DNA. The standard curves were obtained by linear regression analysis. In the case of the cloned 111 bp fragment of SV40 large T antigen the standard SV40 DNA dilutions were made with and without the addition of human genomic DNA isolated from PBMNC from healthy individuals to give a concentration of human genomic DNA of 1 lg/50 ll per assay. This did not lead to changes of the standard curves (one example is shown in this figure).
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    FIGURE 1 – Standard curves for quanti- tative real-time PCR (TaqMan) assays for SV40 large T antigen, EBV-BKRF-1 (EBNA-1), human K-ras and t(14;18). Different linearized plasmid DNAs con- taining the cloned target DNA fragments or the complete SV40 viral genome were 2-fold serial diluted (105 copies to <1 copy) and 3 replicates were tested. Mean cycle threshold values (CT) of 3 replicate tests were plotted against the log copy number of each gene or the cloned viral DNA. The standard curves were obtained by linear regression analysis. In the case of the cloned 111 bp fragment of SV40 large T antigen the standard SV40 DNA dilutions were made with and without the addition of human genomic DNA isolated from PBMNC from healthy individuals to give a concentration of human genomic DNA of 1 lg/50 ll per assay. This did not lead to changes of the standard curves (one example is shown in this figure).

    Journal: International journal of cancer

    Article Title: No evidence for simian virus 40 DNA sequences in malignant non-Hodgkin lymphomas.

    doi: 10.1002/ijc.21346

    Figure Lengend Snippet: FIGURE 1 – Standard curves for quanti- tative real-time PCR (TaqMan) assays for SV40 large T antigen, EBV-BKRF-1 (EBNA-1), human K-ras and t(14;18). Different linearized plasmid DNAs con- taining the cloned target DNA fragments or the complete SV40 viral genome were 2-fold serial diluted (105 copies to <1 copy) and 3 replicates were tested. Mean cycle threshold values (CT) of 3 replicate tests were plotted against the log copy number of each gene or the cloned viral DNA. The standard curves were obtained by linear regression analysis. In the case of the cloned 111 bp fragment of SV40 large T antigen the standard SV40 DNA dilutions were made with and without the addition of human genomic DNA isolated from PBMNC from healthy individuals to give a concentration of human genomic DNA of 1 lg/50 ll per assay. This did not lead to changes of the standard curves (one example is shown in this figure).

    Article Snippet: In the case of SV40 a linearized cloned viral DNA (strain pUCS40-CPC/MEN-1 [ATCC catalog No. VRMC-4]) as well as a cloned amplification DNA fragment were used as standards for quantification.

    Techniques: Real-time Polymerase Chain Reaction, Plasmid Preparation, Clone Assay, Isolation, Concentration Assay